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Biotechnology and Biomedical Engineering Immunology Virtual Lab I Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

SANDWICH Elisa

Choose difficulty:

Beginner

Intermediate

Advanced

1. A positive result from sandwich ELISA indicates that the patient's serum contains:

a: Specific antibody Explanation

Explanation

b: Specific antigen Explanation

Explanation

c: Cytokines Explanation

Explanation

d: None of the above Explanation

Explanation

2. An ELISA can be used for:

a: quantitative analysis Explanation

Explanation

b: size analysis Explanation

Explanation

c: absorbance measurements Explanation

Explanation

d: All of the above Explanation

Explanation

3.Which is not true for a blocking buffer solution ?

a: binds to all potential sites of nonspecific interaction Explanation

Explanation

b: improves the sensitivity of an assay by reducing background signal Explanation

Explanation

c: improves the signal-to-noise ratio Explanation

Explanation

d: alters or obscures the epitope for antibody binding Explanation

Explanation

4. Washing step can be performed with a physiologic buffer such as Tris-buffered saline (TBS) or phosphate-buffered saline (PBS):

a: True Explanation

Explanation

b: False Explanation

Explanation

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