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Biotechnology and Biomedical Engineering Immunology Virtual Lab I Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

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Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

INDIRECT Elisa

Choose difficulty:

Beginner

Intermediate

Advanced

1. The substrate of Horse radish peroxidase is:

a: p-nitro phenyl phosphate (NPP) Explanation

Explanation

b: 4-methylumbelliferyl phosphate (MUP) Explanation

Explanation

c: TMB Explanation

Explanation

d: None of the above Explanation

Explanation

2. Indirect ELISA detects the presence of antigen in patient sera:

a: True Explanation

Explanation

b: False Explanation

Explanation

3. The indirect ELISA requires known

a: Antigen Explanation

Explanation

b: Antiody Explanation

Explanation

c: Enzyme Explanation

Explanation

d: Patient sera Explanation

Explanation

4. In indirect ELISA test, the order in which reagents are to be added is 1- enzyme-linked antibody 2- known antigen 3 - patient serum 4 - substrate:

a: 3-2-1-4 Explanation

Explanation

b: 2-4-1-3 Explanation

Explanation

c: 2-3-1-4 Explanation

Explanation

d: 4-2-1-3 Explanation

Explanation

5. In the indirect ELISA test the enzyme-linked antibody will attach to:

a: blocking buffer Explanation

Explanation

b: variable region of the patient antibody Explanation

Explanation

c: constant region of the patient antibody Explanation

Explanation

d: patient antigen Explanation

Explanation

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