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Biotechnology and Biomedical Engineering Immunology Virtual Lab I Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

DIRECT Elisa

Choose difficulty:

Beginner

Intermediate

Advanced

1. Which is not true for a blocking buffer solution ?

a: Binds to all potential sites of nonspecific interaction Explanation

Explanation

b: Improves the sensitivity of an assay by reducing background signal Explanation

Explanation

c: Improving the signal-to-noise ratio Explanation

Explanation

d: Alters or obscures the epitope for antibody binding Explanation

Explanation

2. The major disadvantage of Direct ELISA is:

a: Cross-reactivity of secondary antibody is eliminated Explanation

Explanation

b: Cross-reactivity might occur with the secondary antibody, resulting in nonspecific signal Explanation

Explanation

c: Labeling primary antibodies for each specific ELISA system is time-consuming and expensive Explanation

Explanation

d: Only one antibody and fewer steps are used Explanation

Explanation

3.Direct ELISA is used for the detection of:

a: Soluble antibody Explanation

Explanation

b: Soluble antigen Explanation

Explanation

c: Complement Explanation

Explanation

d: All of the above Explanation

Explanation

4. Washing step can be performed with a physiologic buffer such as tris-buffered saline (TBS) or phosphate-buffered saline (PBS)

a: True Explanation

Explanation

b: False. Explanation

Explanation

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